Journal: Biomicrofluidics

Article Title: Monitoring bacterial biofilms with a microfluidic flow chip designed for imaging with white-light interferometry

doi: 10.1063/1.4985773

Figure Lengend Snippet: Diagram of the cross-section of the flow cell when air is inserted into the viewing chamber for imaging. Light from the microscope passes through the top cover glass and through the biofilm. Light reflected from the biofilm/air bubble interface returns to the microscope and creates an interference pattern on the camera when it combines with light reflected from a reference arm in the objective lens. A vertical scanning range is selected to avoid light reflected from the top surface of the coverglass and any lower interfaces. This configuration allows for non-destructive imaging of a biofilm attached to the top cover glass. Biofilm on the opposing surface can be imaged by inverting the flow cell.

Article Snippet: 17 The viewing windows were 25 mm by 50 mm No. 2 microscope cover glass that is 190 μ m to 250 μ m thick (Ted Pella).

Techniques: Imaging, Microscopy

Journal: Bio-protocol

Article Title: Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

doi: 10.21769/BioProtoc.3344

Figure Lengend Snippet: A. Maize seedling that was used here as sample material. B. A stretch of epidermis (arrow) is peeled from the leaf using forceps and cut out together with a small piece of leaf. C. Sample submerged in FM4-64 solution. D. Sample washed in PBS in a staining jar. E. Sample mounted on a large coverslip on which a central groove was created by taping the edges. F. Sample after application of a small coverslip. G. Change of quencher solution without removal of the sample from the microscope.

Article Snippet: Pipette tips Microscope cover slips, 50 x 20 mm and 20 x 20 mm (CITOTEST, P/N: 80340-0630, 80340-3610) Microscope slide (CITOTEST, P/N: 80320-2140) 15 ml round bottom tubes 10 cm glass culture dish 1.5 ml Centrifuge tube Tape Razor blades Filter paper Yeast ( Saccharomyces cerevisiae , BY4742) Arabidopsis seedlings (Col-0) Maize ( Zea mays , B73) FM4-64 (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Thermo Fisher Scientific, catalog number: T3166) DMSO (Dimethyl sulfoxide) (MP Biomedicals, catalog number: 196055) Trypan blue (Solarbio, catalog number: T8070) Murashige and Skoog (MS) salts (Phyto Technology Laboratories, catalog number: M519) Sucrose (Guangzhou Jinhuada Chemical Reagent, CAS: 57-50-1) Agar (Solarbio, catalog number: A8190) Tryptone (OXIOD, catalog number: LP0042) Yeast extract (OXIOD, catalog number: LP0021) NaCl (Guangzhou Jinhuada Chemical Reagent, CAS: 7647-14-5) Glucose (Guangzhou Jinhuada Chemical Reagent, CAS: 58367-01-4) Na 2 HPO 4 (Guangzhou Jinhuada Chemical Reagent, CAS: 10039-32-4) KH 2 PO 4 (Guangzhou Jinhuada Chemical Reagent, CAS: 7778-77-0) KCl (Guangzhou Jinhuada Chemical Reagent, CAS: 7447-40-7) KOH YDP medium (see Recipes) Half strength MS medium (see Recipes) PBS solution (pH 7.2-7.4) (see Recipes) FM4-64 stock solution (see Recipes) Trypan blue stock solution (see Recipes)

Techniques: Staining, Microscopy